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intercellular cell adhesion molecule 1 icam 1  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology intercellular cell adhesion molecule 1 icam 1
    Figure 1. PCAF is highly expressed in coronary arteries of elderly patients and human atherosclerotic vessels. (A) All specimens were obtained from the proximal right coronary artery of the patient, and the expression of PCAF in human coronary arteries was detected by immunochemistry. The representative slides are shown; the scale bar is 1 mm, 2×. (B) The relationship between patient age and PCAF expression in human coronary arteries (n = 44). (C,D) The coronary intima produced “atherosclerotic plaque” as the criterion for judging atherosclerosis (AS). Western blot detects PCAF expression and aging-related protein (p53, p21, <t>p16,</t> <t>ICAM-1</t> and VCAM-1) levels in human coronary arteries (n = 3). (E,F) Nrf2 signal pathway related protein level was detected by western blot (n = 3). ** p < 0.01 vs. the control group.
    Intercellular Cell Adhesion Molecule 1 Icam 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 4732 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Downregulation of P300/CBP-Associated Factor Protects from Vascular Aging via Nrf2 Signal Pathway Activation."

    Article Title: Downregulation of P300/CBP-Associated Factor Protects from Vascular Aging via Nrf2 Signal Pathway Activation.

    Journal: International journal of molecular sciences

    doi: 10.3390/ijms232012574

    Figure 1. PCAF is highly expressed in coronary arteries of elderly patients and human atherosclerotic vessels. (A) All specimens were obtained from the proximal right coronary artery of the patient, and the expression of PCAF in human coronary arteries was detected by immunochemistry. The representative slides are shown; the scale bar is 1 mm, 2×. (B) The relationship between patient age and PCAF expression in human coronary arteries (n = 44). (C,D) The coronary intima produced “atherosclerotic plaque” as the criterion for judging atherosclerosis (AS). Western blot detects PCAF expression and aging-related protein (p53, p21, p16, ICAM-1 and VCAM-1) levels in human coronary arteries (n = 3). (E,F) Nrf2 signal pathway related protein level was detected by western blot (n = 3). ** p < 0.01 vs. the control group.
    Figure Legend Snippet: Figure 1. PCAF is highly expressed in coronary arteries of elderly patients and human atherosclerotic vessels. (A) All specimens were obtained from the proximal right coronary artery of the patient, and the expression of PCAF in human coronary arteries was detected by immunochemistry. The representative slides are shown; the scale bar is 1 mm, 2×. (B) The relationship between patient age and PCAF expression in human coronary arteries (n = 44). (C,D) The coronary intima produced “atherosclerotic plaque” as the criterion for judging atherosclerosis (AS). Western blot detects PCAF expression and aging-related protein (p53, p21, p16, ICAM-1 and VCAM-1) levels in human coronary arteries (n = 3). (E,F) Nrf2 signal pathway related protein level was detected by western blot (n = 3). ** p < 0.01 vs. the control group.

    Techniques Used: Expressing, Produced, Western Blot, Control

    Figure 2. Downregulation of PCAF inhibits AngII-induced senescence of ECs. (A,B) Western blot to determine the expression of PCAF in each group (n = 6). (C,D) β-galactosidase staining to detect senescence of ECs; Senescent VSMCs were marked in blue, the scale bar is 100 µm, 400× (n = 5). (E,F) p53, p21, p16, ICAM-1 and VCAM-1 protein expression (n = 6). (G) Detection of PCAF (green) and p21 (red) levels by immunofluorescence double staining; the scale bar is 100 µm, 400× (n = 3). ** p < 0.01 vs. the control group; # p < 0.05, ## p < 0.01 vs. the AngII induction with Ad-GFP group (AngII + Ad-GFP group).
    Figure Legend Snippet: Figure 2. Downregulation of PCAF inhibits AngII-induced senescence of ECs. (A,B) Western blot to determine the expression of PCAF in each group (n = 6). (C,D) β-galactosidase staining to detect senescence of ECs; Senescent VSMCs were marked in blue, the scale bar is 100 µm, 400× (n = 5). (E,F) p53, p21, p16, ICAM-1 and VCAM-1 protein expression (n = 6). (G) Detection of PCAF (green) and p21 (red) levels by immunofluorescence double staining; the scale bar is 100 µm, 400× (n = 3). ** p < 0.01 vs. the control group; # p < 0.05, ## p < 0.01 vs. the AngII induction with Ad-GFP group (AngII + Ad-GFP group).

    Techniques Used: Western Blot, Expressing, Staining, Double Staining, Control

    Figure 3. The PCAF inhibitor garcinol significantly attenuates the AngII-induced vascular ag- ing phenotype in mice. (A) H&E staining to observe the vascular structure—the scale bar is 100 µm, 100× (n = 3); immunofluorescence staining to detect the expression of PCAF (green) and p53 (red)—the scale bar is 100 µm, 200× (n = 3). (B) Detection of p16 (red) and p21 (green) expression in mice thoracic aorta by double immunofluorescence staining; the scale bar is 100 µm, 400× (n = 3). (C,D) Western blot detects the expression of PCAF, aging-related proteins (p53, p21, p16, VCAM-1 and ICAM-1) and Nrf2 signaling pathway proteins in mice thoracic aorta (n = 6). ** p < 0.01 vs. the Sham group; # p < 0.05, ## p < 0.01 vs. AngII group.
    Figure Legend Snippet: Figure 3. The PCAF inhibitor garcinol significantly attenuates the AngII-induced vascular ag- ing phenotype in mice. (A) H&E staining to observe the vascular structure—the scale bar is 100 µm, 100× (n = 3); immunofluorescence staining to detect the expression of PCAF (green) and p53 (red)—the scale bar is 100 µm, 200× (n = 3). (B) Detection of p16 (red) and p21 (green) expression in mice thoracic aorta by double immunofluorescence staining; the scale bar is 100 µm, 400× (n = 3). (C,D) Western blot detects the expression of PCAF, aging-related proteins (p53, p21, p16, VCAM-1 and ICAM-1) and Nrf2 signaling pathway proteins in mice thoracic aorta (n = 6). ** p < 0.01 vs. the Sham group; # p < 0.05, ## p < 0.01 vs. AngII group.

    Techniques Used: Staining, Expressing, Western Blot

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    Article Snippet: .. Immunostained sections were preincubated with 5% normal goat serum and then incubated with anti-proliferating cell nuclear antigen (PCNA) (1:1000, ab92552, Abcam), anti-NFp65 (phospho S536) (1:500, ab28856, Abcam), anti-intercellular cell adhesion molecule (ICAM)-1 (1:200, sc-8439, Santa Cruz), antivascular cell adhesion molecule (VCAM)-1 (1:200, sc-8304, Santa Cruz) anti-CD14 (1:500 dilution, ab182032, Abcam), and antiCD68 (1:500 dilution, ab955, Abcam) antibody. .. The sections were incubated with the horseradish peroxidase streptavidin biotinylated secondary antibody (Dako) followed by diaminobenzidine (DAB kit, Vector Laboratories) and counter stained with Gill’s haematoxylin (Fisher Scientific).

    Article Title: CKII-SIRT1-SM22α loop evokes a self-limited inflammatory response in vascular smooth muscle cells.
    Article Snippet: .. Immunostaining of sections were preincubated with 5% normal goat serum and then incubated with anti-monocyte chemotactic protein (MCP)-1 (1:400, Santa Cruz), anti-matrix metalloproteinase (MMP)2 (1:200, sc-13595, Santa Cruz), anti-MMP-9 (1:200, sc-6840, Santa Cruz), anti-intercellular cell adhesion molecule (ICAM)-1 (1:200, sc-8439, Santa Cruz), and anti-vascular cell adhesion molecule (VCAM)-1 (1:200, sc-8304, Santa Cruz) antibody. .. The sections were incubated with the horseradish peroxidase streptavidin biotinylated secondary antibody (Dako) followed by diaminobenzidine (DAB kit, Vector Laboratories) and counterstained with Gill’s haematoxylin (Fisher Scientific).

    Immunostaining:

    Article Title: CKII-SIRT1-SM22α loop evokes a self-limited inflammatory response in vascular smooth muscle cells.
    Article Snippet: .. Immunostaining of sections were preincubated with 5% normal goat serum and then incubated with anti-monocyte chemotactic protein (MCP)-1 (1:400, Santa Cruz), anti-matrix metalloproteinase (MMP)2 (1:200, sc-13595, Santa Cruz), anti-MMP-9 (1:200, sc-6840, Santa Cruz), anti-intercellular cell adhesion molecule (ICAM)-1 (1:200, sc-8439, Santa Cruz), and anti-vascular cell adhesion molecule (VCAM)-1 (1:200, sc-8304, Santa Cruz) antibody. .. The sections were incubated with the horseradish peroxidase streptavidin biotinylated secondary antibody (Dako) followed by diaminobenzidine (DAB kit, Vector Laboratories) and counterstained with Gill’s haematoxylin (Fisher Scientific).



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    Figure 1. PCAF is highly expressed in coronary arteries of elderly patients and human atherosclerotic vessels. (A) All specimens were obtained from the proximal right coronary artery of the patient, and the expression of PCAF in human coronary arteries was detected by immunochemistry. The representative slides are shown; the scale bar is 1 mm, 2×. (B) The relationship between patient age and PCAF expression in human coronary arteries (n = 44). (C,D) The coronary intima produced “atherosclerotic plaque” as the criterion for judging atherosclerosis (AS). Western blot detects PCAF expression and aging-related protein (p53, p21, <t>p16,</t> <t>ICAM-1</t> and VCAM-1) levels in human coronary arteries (n = 3). (E,F) Nrf2 signal pathway related protein level was detected by western blot (n = 3). ** p < 0.01 vs. the control group.
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    Figure 1. PCAF is highly expressed in coronary arteries of elderly patients and human atherosclerotic vessels. (A) All specimens were obtained from the proximal right coronary artery of the patient, and the expression of PCAF in human coronary arteries was detected by immunochemistry. The representative slides are shown; the scale bar is 1 mm, 2×. (B) The relationship between patient age and PCAF expression in human coronary arteries (n = 44). (C,D) The coronary intima produced “atherosclerotic plaque” as the criterion for judging atherosclerosis (AS). Western blot detects PCAF expression and aging-related protein (p53, p21, <t>p16,</t> <t>ICAM-1</t> and VCAM-1) levels in human coronary arteries (n = 3). (E,F) Nrf2 signal pathway related protein level was detected by western blot (n = 3). ** p < 0.01 vs. the control group.
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    Image Search Results


    Figure 1. PCAF is highly expressed in coronary arteries of elderly patients and human atherosclerotic vessels. (A) All specimens were obtained from the proximal right coronary artery of the patient, and the expression of PCAF in human coronary arteries was detected by immunochemistry. The representative slides are shown; the scale bar is 1 mm, 2×. (B) The relationship between patient age and PCAF expression in human coronary arteries (n = 44). (C,D) The coronary intima produced “atherosclerotic plaque” as the criterion for judging atherosclerosis (AS). Western blot detects PCAF expression and aging-related protein (p53, p21, p16, ICAM-1 and VCAM-1) levels in human coronary arteries (n = 3). (E,F) Nrf2 signal pathway related protein level was detected by western blot (n = 3). ** p < 0.01 vs. the control group.

    Journal: International journal of molecular sciences

    Article Title: Downregulation of P300/CBP-Associated Factor Protects from Vascular Aging via Nrf2 Signal Pathway Activation.

    doi: 10.3390/ijms232012574

    Figure Lengend Snippet: Figure 1. PCAF is highly expressed in coronary arteries of elderly patients and human atherosclerotic vessels. (A) All specimens were obtained from the proximal right coronary artery of the patient, and the expression of PCAF in human coronary arteries was detected by immunochemistry. The representative slides are shown; the scale bar is 1 mm, 2×. (B) The relationship between patient age and PCAF expression in human coronary arteries (n = 44). (C,D) The coronary intima produced “atherosclerotic plaque” as the criterion for judging atherosclerosis (AS). Western blot detects PCAF expression and aging-related protein (p53, p21, p16, ICAM-1 and VCAM-1) levels in human coronary arteries (n = 3). (E,F) Nrf2 signal pathway related protein level was detected by western blot (n = 3). ** p < 0.01 vs. the control group.

    Article Snippet: Antibodies against PCAF (#sc-13124), NAD(P)H quinone oxidoreductase-1 (#sc-376023), Cyclin-dependent kinase inhibitor 1A (p21) (#sc-6246), cyclin-dependent kinase inhibitor 2A (p16) (#sc-1661), intercellular cell adhesion molecule-1 (ICAM-1) (#sc-8439) and vascular cell adhesion molecule 1 (VCAM-1) (#sc-13160) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Expressing, Produced, Western Blot, Control

    Figure 2. Downregulation of PCAF inhibits AngII-induced senescence of ECs. (A,B) Western blot to determine the expression of PCAF in each group (n = 6). (C,D) β-galactosidase staining to detect senescence of ECs; Senescent VSMCs were marked in blue, the scale bar is 100 µm, 400× (n = 5). (E,F) p53, p21, p16, ICAM-1 and VCAM-1 protein expression (n = 6). (G) Detection of PCAF (green) and p21 (red) levels by immunofluorescence double staining; the scale bar is 100 µm, 400× (n = 3). ** p < 0.01 vs. the control group; # p < 0.05, ## p < 0.01 vs. the AngII induction with Ad-GFP group (AngII + Ad-GFP group).

    Journal: International journal of molecular sciences

    Article Title: Downregulation of P300/CBP-Associated Factor Protects from Vascular Aging via Nrf2 Signal Pathway Activation.

    doi: 10.3390/ijms232012574

    Figure Lengend Snippet: Figure 2. Downregulation of PCAF inhibits AngII-induced senescence of ECs. (A,B) Western blot to determine the expression of PCAF in each group (n = 6). (C,D) β-galactosidase staining to detect senescence of ECs; Senescent VSMCs were marked in blue, the scale bar is 100 µm, 400× (n = 5). (E,F) p53, p21, p16, ICAM-1 and VCAM-1 protein expression (n = 6). (G) Detection of PCAF (green) and p21 (red) levels by immunofluorescence double staining; the scale bar is 100 µm, 400× (n = 3). ** p < 0.01 vs. the control group; # p < 0.05, ## p < 0.01 vs. the AngII induction with Ad-GFP group (AngII + Ad-GFP group).

    Article Snippet: Antibodies against PCAF (#sc-13124), NAD(P)H quinone oxidoreductase-1 (#sc-376023), Cyclin-dependent kinase inhibitor 1A (p21) (#sc-6246), cyclin-dependent kinase inhibitor 2A (p16) (#sc-1661), intercellular cell adhesion molecule-1 (ICAM-1) (#sc-8439) and vascular cell adhesion molecule 1 (VCAM-1) (#sc-13160) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Western Blot, Expressing, Staining, Double Staining, Control

    Figure 3. The PCAF inhibitor garcinol significantly attenuates the AngII-induced vascular ag- ing phenotype in mice. (A) H&E staining to observe the vascular structure—the scale bar is 100 µm, 100× (n = 3); immunofluorescence staining to detect the expression of PCAF (green) and p53 (red)—the scale bar is 100 µm, 200× (n = 3). (B) Detection of p16 (red) and p21 (green) expression in mice thoracic aorta by double immunofluorescence staining; the scale bar is 100 µm, 400× (n = 3). (C,D) Western blot detects the expression of PCAF, aging-related proteins (p53, p21, p16, VCAM-1 and ICAM-1) and Nrf2 signaling pathway proteins in mice thoracic aorta (n = 6). ** p < 0.01 vs. the Sham group; # p < 0.05, ## p < 0.01 vs. AngII group.

    Journal: International journal of molecular sciences

    Article Title: Downregulation of P300/CBP-Associated Factor Protects from Vascular Aging via Nrf2 Signal Pathway Activation.

    doi: 10.3390/ijms232012574

    Figure Lengend Snippet: Figure 3. The PCAF inhibitor garcinol significantly attenuates the AngII-induced vascular ag- ing phenotype in mice. (A) H&E staining to observe the vascular structure—the scale bar is 100 µm, 100× (n = 3); immunofluorescence staining to detect the expression of PCAF (green) and p53 (red)—the scale bar is 100 µm, 200× (n = 3). (B) Detection of p16 (red) and p21 (green) expression in mice thoracic aorta by double immunofluorescence staining; the scale bar is 100 µm, 400× (n = 3). (C,D) Western blot detects the expression of PCAF, aging-related proteins (p53, p21, p16, VCAM-1 and ICAM-1) and Nrf2 signaling pathway proteins in mice thoracic aorta (n = 6). ** p < 0.01 vs. the Sham group; # p < 0.05, ## p < 0.01 vs. AngII group.

    Article Snippet: Antibodies against PCAF (#sc-13124), NAD(P)H quinone oxidoreductase-1 (#sc-376023), Cyclin-dependent kinase inhibitor 1A (p21) (#sc-6246), cyclin-dependent kinase inhibitor 2A (p16) (#sc-1661), intercellular cell adhesion molecule-1 (ICAM-1) (#sc-8439) and vascular cell adhesion molecule 1 (VCAM-1) (#sc-13160) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Staining, Expressing, Western Blot

    PRDM16 inhibited AS through inactivation of the TGF-β signaling pathway. (A) The content of TC, TG, and HDL-C in peripheral blood in mice in each group as detected by biochemical indicators. (B) The changes of aortic plaques in AS mice as detected by HE staining (×200, scale bar: 50 μm). (C) The number of aortic thrombosis in AS mice as detected by ORO staining (×400, scale bar: 25 μm). (D) Positive rates of AS markers (ICAM-1 and VCAM-1) in the aorta of AS mice as detected by IHC (×400). * p < 0.05 compared with mice treated with DMSO, # p < 0.05 compared with mice treated with oe-PRDM16 NC. The measurement data were expressed as mean ± standard deviation, and analyzed by independent-sample t -test, n = 15.

    Journal: Frontiers in Physiology

    Article Title: PRDM16 Upregulation Induced by MicroRNA-448 Inhibition Alleviates Atherosclerosis via the TGF-β Signaling Pathway Inactivation

    doi: 10.3389/fphys.2020.00846

    Figure Lengend Snippet: PRDM16 inhibited AS through inactivation of the TGF-β signaling pathway. (A) The content of TC, TG, and HDL-C in peripheral blood in mice in each group as detected by biochemical indicators. (B) The changes of aortic plaques in AS mice as detected by HE staining (×200, scale bar: 50 μm). (C) The number of aortic thrombosis in AS mice as detected by ORO staining (×400, scale bar: 25 μm). (D) Positive rates of AS markers (ICAM-1 and VCAM-1) in the aorta of AS mice as detected by IHC (×400). * p < 0.05 compared with mice treated with DMSO, # p < 0.05 compared with mice treated with oe-PRDM16 NC. The measurement data were expressed as mean ± standard deviation, and analyzed by independent-sample t -test, n = 15.

    Article Snippet: The tissue sections were probed with primary antibodies to intercellular cell adhesion molecule-1 (ICAM-1) (#4915, 1:50, Cell Signaling Technology, Danvers, MA, United States) and vascular cell adhesion molecule-1 (VCAM-1) (ab134047, 1:250, Abcam, Cambridge, United Kingdom) at 4°C overnight.

    Techniques: Staining, Standard Deviation